Objectives: To re-evaluate a sample of older adults enrolled in a randomized controlled trial of lithium for amnestic mild cognitive impairment (MCI) after 11 to 15 years, re-assessing their current (or last available) global cognitive and functional state. Methods: We recalled all former participants of the Lithium-MCI trial conducted by our group between 2009 and 2012 to perform a single-blinded, cross-sectional evaluation of their global clinical state to compare the long-term outcome of those who received lithium vs. those who received placebo. Results: Of the original sample (n=61), we were able to reach 36 participants (59% of retention), of whom 22 had previously received lithium (61% of the recall sample) and 14 (39%) had received placebo. Since 30.5% of the recalled sample was deceased, psychometric data were collected only for 69.5% of the participants. We found statistically significant differences in current mean Mini Mental State Examination score according to previous treatment group (25.5 [SD, 5.3] vs. 18.3 [SD, 10.9], p = 0.04). The lithium group also had better performance in the phonemic Verbal Fluency Test than the control group (34.4 [SD, 14.4] vs. 11.6 [SD, 10.10], p < 0.001). Differences in these measures also had large effect sizes, as shown by Cohen’s d values of 0.92 and 1.78, respectively. Conclusion: This data set suggests that older adults with amnestic MCI who had been treated with lithium during a previous randomized controlled trial had a better long-term global cognitive outcome than those from a matched sample who did not receive the intervention.

Abstract This work consists of a case study analysis of the upper basin of the Cuñapirú stream, located in the department of Rivera, in the area known as Puntas de Cuñapirú, identifying environmental problems and issues related to territorial transformations in the period 1990-2017. It aims to provide elements for understanding the processes of territorial transformations over a period of 27 years. The application of an environmental vulnerability model that integrates fragility and threat factors makes it possible to identify areas that are particularly sensitive to current and future impacts and facilitates the identification of critical interactions in the environmental system, generating inputs for decision-makers. The application of the model was able to identify spatially the areas with the highest rate of urban expansion, which in turn constitutes the main threat and generates the highest levels of vulnerability. It was also possible to recognise which natural assets interact with these changes in land use (environmental fragility). It is hoped that the results obtained will help to strengthen land-use planning and management capacities.

Resumen El presente trabajo consiste en un análisis de caso de la cuenca alta de la Arroyo Cuñapirú, ubicada en el departamento de Rivera, en la zona conocida como Puntas de Cuñapirú, identificando las problemáticas ambientales y las cuestiones vinculadas a las transformaciones del territorio, en el periodo 1990-2017. Tiene como objetivo aportar elementos para la comprensión de los procesos de trasformaciones territoriales en un periodo de 27 años. La aplicación de un modelo de vulnerabilidad ambiental que integra factores de fragilidad y amenaza permite identificar áreas que son particularmente sensibles a actuales y futuros impactos y facilita la identificación de interacciones críticas en el sistema ambiental, generando insumos para los tomadores de decisiones. La aplicación del modelo logró identificar en el espacio las zonas con mayor tasa de expansión urbana, la que a su vez constituye la principal amenaza y genera los mayores niveles de vulnerabilidad. También se logró reconocer cuales bienes de la naturaleza interactúan con esos cambios en el uso del suelo (fragilidad ambiental). Se espera que los resultados obtenidos colaboren a fortalecer las capacidades del ordenamiento y gestión territorial.

Abstract The objective of this work was to map the chromosomal regions responsible for iron accumulation in rice grains, in Southern Brazil. Eighty-one rice accessions were genotyped and phenotyped for Fe accumulation. Single nucleotide polymorphisms were mapped in the whole grain on chromosomes 1, 5, 6, and 10, from which 13 candidate genes were identified. Some of the genes, such as O s10g040680 0, seem to have a relationship with Fe homeostasis, while others are related to other metabolic processes or have an unknown function.

Resumo O objetivo deste trabalho foi mapear regiões cromossômicas responsáveis pelo acúmulo de ferro em grãos de arroz, na região Sul do Brasil. Oitenta e um acessos de arroz foram genotipados e fenotipados quanto ao acúmulo de Fe. Polimorfismos de nucleotídeos únicos foram mapeados no grão integral, nos cromossomos 1, 5, 6 e 10, dos quais 13 genes candidatos foram identificados. Alguns dos genes, como o Os10g0406800, parecem ter relação com a homeostase do Fe, enquanto outros têm relação com outros processos metabólicos ou função desconhecida.

Resumo: Este artigo busca promover reflexões sobre a prática de análise linguística (PAL), um dos eixos articuladores do ensino de língua portuguesa no Brasil ( BRASIL, 1998, 2019), na formação docente inicial de professores de língua, com vistas a responder axiologicamente aos documentos político-educacionais brasileiros e aos estudos contemporâneos que ratificam o trabalho com as práticas de linguagem na educação básica alinhado a uma abordagem social e histórica sob o matiz das interações. Em termos teóricos, ancoramo-nos nos estudos dialógicos da linguagem ( BAKHTIN [VOLOCHÍNOV], 2014; BAKHTIN, 2011; VOLOCHÍNOV 2013; MEDVIÉDEV, 2012) e, em termos metodológicos, empreendemos uma análise documental ( BARDIN, 2011; BOWEN, 2009; CELLARD, 2008) ante o universo de dados composto pelo currículo dos cursos de licenciatura em letras das universidades estaduais do Paraná, Brasil. Os resultados enunciam uma presença pouco representativa da PAL como “prática de linguagem” ( GERALDI, 2011) e evidenciam a necessidade

Abstract: This paper seeks to promote reflections on the practice of linguistic analysis (PLA), one of the articulating axes of Portuguese Language Teaching in Brazil ( BRASIL, 1998, 2019), in the initial training of language teachers. With that, it aims to respond axiologically to the Brazilian political-educational documents and contemporary studies that ratify the work with language practices aligned with a sociohistorical approach under the hue of interactions in basic education. To this end, data obtained from the curriculum of Language and Literature teaching courses of the state universities of Paraná-PR underwent documentary analysis ( BARDIN, 2011; BOWEN, 2009; CELLARD, 2008) in the light of the dialogic studies of language ( BAKHTIN [VOLOCHÍNOV], 2014; BAKHTIN, 2011; VOLOCHÍNOV, 2013; MEDVIÉDEV, 2012). The results indicate that PLA is not very representative as a “language practice” ( GERALDI, 2011), highlighting the need for continued reflection on the matter.

Resumo Este estudo apresenta a síntese de cimentos de ionômero de vidro (GIC) modificados com nanopartículas de fosfato de cálcio (nCaP). Os nCaP / GIC foram submetidos a ensaios mecânicos de compressão e tração diametral. Os biocompósitos foram caracterizados por microscopia eletrônica de varredura (MEV), espectroscopia de energia dispersiva de raios-X (EDX), difração de raios-X (XRD) e espectroscopia de infravermelho com transformada de Fourier (FTIR). Os testes de citotoxicidade e viabilidade celular foram realizados em células-tronco mesenquimais da medula óssea humana usando um ensaio de 3- (4,5-dimetiltiazol-2-il) 2,5-difeniltetrazólio-brometo e ensaios LIVE / DEAD. Diferenças estatisticamente significativas foram observadas para as propriedades mecânicas (Kruskal-Wallis, p <0,001), nCaP / GIC apresentou maior resistência à compressão e tração diametral. As análises de SEM revelaram uma distribuição uniforme de nCaP na matriz do ionômero. Os resultados de EDX e DRX indicaram fases de hidroxiapatita e β-trifosfato de cálcio. Os espectros de FTIR revelaram a banda assimétrica de ν3PO4 3- entre 1100-1030cm-1 e a banda de vibração associada a ν1PO4 3- em 963cm-1 associada a nCaP. O nCaP / GIC apresentou resposta adequada à viabilidade celular e comportamento não citotóxico. Portanto, o novo compósito nCaP / GIC apresentou ótimas propriedades mecânicas, comportamento não citotóxico e resposta adequada à viabilidade celular com promissoras aplicações odontológicas.

Abstract This study showed the synthesis of Glass ionomer cements (GIC) modified with calcium phosphate nanoparticles (nCaP). The nCaP/GIC were submitted to mechanical compression and diametral tensile tests. The biocomposite were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR). Cytotoxicity and cell viability tests were performed on the human bone marrow mesenchymal stem cells using a 3-(4,5-dimethylthiazol-2yl)2,5-diphenyl- tetrazolium-bromide assay and LIVE/DEAD assays. Statistically significant differences were observed for mechanical properties (Kruskal-Wallis, p<0.001), nCaP/GIC showed higher resistance to compression and diametral traction. The SEM analyses revealed a uniform distribution nCaP in the ionomer matrix. The EDX and XRD results indicated that hydroxyapatite and calcium β-triphosphate phases. The FTIR spectra revealed the asymmetric band of ν3PO43- between 1100-1030cm-1 and the vibration band associated with ν1PO43- in 963cm-1 associated with nCaP. The nCaP/GIC presented response to adequate cell viability and non-cytotoxic behavior. Therefore, the new nCaP/GIC composite showed great mechanical properties, non-cytotoxic behavior, and adequate response to cell viability with promising dental applications.

ABSTRACT: Several non-destructive testing techniques have been improved and tested in recent years. The non-destructive testing techniques using particle image velocimetry (PIV) and digital image correlation (DIC) differ from conventional testing techniques, as they allow making measurements indirectly. In this study, the elastic modulus in wood beams of Eucalyptus grandis and Pinus oocarpa were determined using PIV and DIC. The application of PIV and DIC techniques occurred during the static bending tests, when the displacements were also measured by the conventional method. The applied load values allowed calculating the elasticity modulus. In all regions of analysis, the mean values of the elasticity modulus found by the DIC, PIV and conventional method are statistically equivalent. It is concluded that the PIV and DIC testing techniques can be used to determine mechanical properties of wood.

Abstract: Non-destructive techniques for characterizing materials in-service have been increasing in importance. Thus, it is relevant to assess the potential of non-destructive techniques for solid materials. This work aimed to determine the modulus of elasticity of Bertholletia excelsa wood using the particle image velocimetry technique and the ultrasound method to compare the results with the conventional methodology. For this purpose, samples of Bertholletia excelsa were made using a circular saw. The samples were evaluated for sound propagation to calculate the modulus of elasticity using ultrasound equipment. Subsequently, they were subjected to the compression parallel to grain test in a universal testing machine. The samples were marked and monitored during the loading session, with the repeated capture of images using a professional camera. The deformation values obtained were used to estimate the modulus of elasticity using the particle image velocimetry technique. The mean values of modulus of elasticity found were 17403 MPa for ultrasound, 15589 MPa for the particle image velocimetry technique, and 15333 MPa for the universal testing machine. The particle image velocimetry technique was considered to be statistically similar (Tukey α = 0,05) to the other methods tested. The linear coefficient of determination (R2) between the particle image velocimetry technique and the universal testing machine was 0,95, a high and satisfactory value. Thus, the particle image velocimetry technique and the ultrasound method are valid to estimate the modulus of elasticity of Bertholletia excelsa wood and possibly of woods with similar technological characteristics.

Resumo Introdução As próteses parafusadas possuem orifício de acesso ao parafuso (SAH), os quais são selados com resina composta. Sua perda ou desgaste está entre as complicações mais comuns em próteses sobre implantes, associadas a fratura da lâmina cerâmica. Objetivo Assim, é importante avaliar a influência dos materiais de selamento na resistência à fratura de resina composta aplicada ao SAH no selamento de prótese sobre implantes parafusadas. Material e método As amostras foram produzidas utilizando pilares UCLA em liga metálica de NiCr com posterior aplicação de cerâmica. Após asperização e condicionamento da superfície cerâmica, foram aplicados silano e adesivo dentinário, antes da selagem dos condutos com as resinas compostas Z100 e P60. Foram avaliados nove grupos (n = 10): selamento com Z-100 (ZNC) e P-60 (PNC) sem selamento do SAH; selamento com Z100 (ZCP) e P-60 (PCP) com algodão absorvente; Z100 (ZPT) e P60 (PPT) com politetrafluoroetileno; Z100 (ZGP) e P60 (PGP) com guta-percha e coroa de cerâmica cimentada (ICS). Após o teste de resistência à fratura, os dados foram analisados usando ANOVA de dois fatores e testes Tukey HSD (p<0,05). O tipo de fratura foi avaliado por microscópio eletrônico de varredura. Resultado Independentemente do material obturador, os maiores valores médios de resistência à fratura foram observados no selamento com P60 (p=0,002). Quando combinados com resinas compostas por um material de selamento, os resultados obtidos foram: ZGP: 805,5N/ PGP: 929,5N<ZPT: 1079,1N/ PPT: 1149,5N=ZNC 1183,1N/ PNC: 1350,6N <ZCP: 1403,6N / PCP: 1641,3N <ICS: 2645,0N. Conclusão O uso de P60 com algodão exibiu a maior resistência à fratura.

Abstract Introduction Screw-retained restorations have a screw access hole (SAH) sealed with resin composite aiming at safe-guarding the aesthetic features of the ceramic veneer. The loss or wear of the resin composite applied in sealing the SAH is among the most common complications in implant prosthodontics, as the fracture of ceramic veneer. Objective Evaluate the influence of sealant materials on the fracture resistance of resin composite applied in sealing screw access hole in screwed (SAH) implants. Material and method The samples were produced from UCLA abutments in metallic NiCr alloy with subsequent application of ceramic. After asperisation and conditioning ceramic surface, was applied silane and dentin adhesive, before sealing the conduits with resin composites Z100 and P60. Nine groups (n=10) were evaluated: sealing with Z-100 (ZNC) and P-60 (PNC) without obturation of SAH; sealing with Z100 (ZCP) and P-60 (PCP) with absorbent cotton; Z100 (ZPT) and P60 (PPT) with polytetrafluoroethylene; Z100 (ZGP) and P60 (PGP) with gutta-percha and a cemented ceramic crown (ICS). After the fracture resistance test, the data were analyzed using two-way ANOVA and Tukey HSD tests (p<.05). Result The fracture mode was evaluated by scanning electron microscope. Irrespective of the filling material, the highest mean values of fracture resistance were observed in the sealing with P60 (p=.002). When combined with resins composed of a sealing material, the results obtained were: ZGP: 805.5N/ PGP: 929.5N<ZPT: 1079.1N/ PPT: 1149.5N=ZNC 1183.1N/ PNC: 1350.6N<ZCP: 1403.6N/ PCP: 1641.3N<ICS: 2645.0N. Conclusion The use of P60 with cotton wool exhibited the highest fracture resistance.

ABSTRACT Ficus inflorescences host a species-rich chalcid wasp community, including pollinating fig wasps (Agaonidae: Tetrapusinae, Kradibiinae, and Agaoninae) and several species of non-pollinating fig wasps (NPFW), that show several life-history strategies, including gall-inducers, kleptoparasites (i. e. inquilines), and parasitoids. We analyzed the structure and degree of specialization of the fig wasp community associated with Ficus inflorescences in urbanized areas, agroecosystems, and on the edge of forest fragments in the state of Goiás (Brazil). We sampled 34 wasp species in four native Ficus tree species, from which four wasp species occurred in more than one host. Neotropical fig pollinators (Pegoscapus and Tetrapus) were the most abundant species, and they were host-specific, although two pollinator species were associated with Ficus obtusifolia. The Jaccard similarity index was higher in samples of fig wasps collected in the same host, indicating that the community composition was specific to each host species. Community structure indices indicate a specialized structure with low connectance, high bidimensional Shannon H2’ and low partner diversity. The communities present a modular web structure in which modules were represented by each host and its associated insect species. These results indicate that the fig wasp communities analyzed are highly specialized, despite a few not strictly host-specific species.

Abstract Listeria monocytogenes is a food pathogen responsible for many foodborne disease outbreaks. Listeria innocua is similar to L. monocytogenes, could be considered biologically similar to L. monocytogenes and could be used to predict its behavior. The aims of this work were to isolate Listeria spp. in dairy processing plants, perform genotypic characterization of virulence, evaluate the antimicrobial resistance profile, and verify the sensitivity of the strains to essential oils. Sampling was held in six dairy processing plants in the region of Campinas, Brazil. Seventeen Listeria innocua strains were isolated, all negative for virulence genes inlA, inlB, inlC, inlJ, actA, plcA, and plcB. The strains were resistant to kanamycin (5.9%), penicillin (11.8%), ampicillin (11.8%), cefoxitin (58.8%), clindamycin (76.50%) and oxacillin (100%). The susceptibility of the strains to commercial essential oils (Rosemarinus officinalis, Cymbopogon citratus, Eucalyptus citirodora, Mentha piperita, Piper nigrum, Vetiveria zizanioides, Cymbopogon nardus, Cymbopogon martinii and Pogostemon cablin) was evaluated by Minimum Inhibitory Concentration (MIC) test. The results showed Cymbopogon citratus, Cymbopogon martini, Cymbopogon nardus and Mentha piperita oils were most efficient. The resistance of Listeria innocua isolated in dairy processing plants is worrisome, and the use of essential oils could be an alternative for development of new drugs and products.

This paper builds a real business cycle (RBC) model with government, aiming to replicate key features of the Brazilian economy. I first calibrate and then I use Bayesian methods to estimate the model for Brazil, with 20 years of quarterly aggregate data. Contrary to the conventional knowledge, I find mixed evidence on the pro-cyclicality of the fiscal policy in Brazil. Moreover, the results suggest that tax rate changes have been used to counter changes in the level of government indebtedness, however in a small degree if compared to other international evidence.

Abstract Objective The aim of this study is to evaluate the new bone and connective tissue formation and the biomaterial remaining after maxillary sinus bone augmentation using 5 different bone substitutes. The osteocalcin immunolabeling was performed to demonstrate their calcification and the possibility of receiving dental implants. Methodology 40 patients underwent maxillary sinus bone augmentation and were divided in 5 groups: Group 1 with 8 maxillary sinuses were grafted with autogenous bone graft (AB); Group 2 with 8 maxillary sinuses grafted with bioactive glass (BG); Group 3 with 8 maxillary sinuses grafted with bioactive glass added to autogenous bone graft (BG + AB) 1:1; Group 4 with 8 maxillary sinuses grafted with Bio-Oss (BO) and Group 5 with 8 maxillary sinuses grafted with Bio-Oss added to autogenous bone graft (BO + AB) 1:1. Results In group AB, 37.8% of bone was formed in the pristine bone region, 38.1% in the intermediate and 44.5% in the apical region. In group BG, 43.6% was formed in the pristine bone, 37% in the intermediate and 49.3% in the apical region. In group BG + AB 1:1, 39.0% was formed in the pristine bone region, 34.8% in the intermediate and 36.8% in apical region. In group BO, 33.4% was formed in the pristine bone, 32.5% in the intermediate and 34.3% in the apical region. In group BO + AB 1:1, 32.8% was formed in the pristine bone, 36.1% in intermediate and 27.8% in the apical regions. The immunolabeling for osteocalcin showed an intensive staining for all groups, which could demonstrate the calcification of the bone formed. Conclusion This study showed that the groups evaluated formed a suitable lamellar bone in the maxillary sinus reconstruction after six months of bone healing, thus being indicated to receive dental implants.

Abstract: The aim of this work was to verify the ability to use the Particle Image Velocimetry technique for measurements of dimensional variations resulting from wood retractability of Pinus elliottii wood, initially saturated and with the surface marked with multiple dots of ink randomly distributed, was used in this work to apply the Particle Image Velocimetry technique. The specimens were dried and images were captured during the process. The images obtained were processed by the Particle Image Velocimetry algorithm and the deformations that occurred were calculated. For comparison, a conventional method (pachymeter) was used to measure the dimensions of the specimen during drying. The variation in dimensions obtained on the surface of the specimens from the Particle Image Velocimetry technique was 2,28 % for the radial direction and 0,20 % for the longitudinal direction of the fibers. With the standardized method, these values were 2,18 % for the radial direction and 0,21 % for longitudinal. The reduction in the average area of the specimens was 3,85 % by the Particle Image Velocimetry technique and 3,77 % by the conventional methodology. It was concluded that the Particle Image Velocimetry technique was able to accurately measure the displacements on the surface of the Pinus elliottii specimens, resulting in values statistically similar to those reached through the use of the conventional measurement method, demonstrating its reliability.

Discute-se um experimento didático que envolve conceitos e leis da ótica (p. ex., ângulo de Brewster, equações de Fresnel, polarização) inseridos em uma atividade com enfoque no processo de modelagem científica concebida para aulas de laboratório de cursos de graduação em Física. A atividade é motivada por uma situação-problema que envolve um aparente mistério relacionado com o desaparecimento de um objeto nas profundezas de um lago de águas cristalinas. Para desvendar o aparente mistério, é aventado que a reflexão e transmissão da luz solar pela superfície do lago têm papel determinante na explicação do fenômeno. Um modelo teórico é, então, construído e avaliado por meio de um experimento em que a luz de um laser, polarizada na direção paralela e perpendicular ao plano de incidência, é refletida na superfície da água contida em uma cuba. Mostra-se que esse modelo é capaz de prever o desaparecimento, ao longo da tarde, de uma moeda contida em uma cuba com um pouco de água e iluminados pela luz solar. Entendemos que o presente artigo contribui para o ensino de Física na medida em que traz uma atividade que fomenta a vinculação entre conhecimentos teóricos e práticos a partir de um problema potencialmente instigante, contribuindo para que os estudantes construam sentido para conhecimentos científicos e compreendam elementos centrais do trabalho experimental da perspectiva da modelagem científica.

A didactic experiment involving concepts and laws of optics (e.g., Brewster angle, Fresnel equations, polarization) is inserted in an activity focusing on the scientific modeling process designed for laboratory classes in an undergraduate Physics course. The activity is motivated by a problem that involves an apparent mystery related to the disappearance of an object in the dephts of a crystal clear lake. To unravel the apparent mystery, it is suggested that the reflection and transmission of sunlight across the lake’s surface has a determining role in explaining the phenomenon. A theoretical model is then built and evaluated by means of an experiment in which a laser light, polarized in the parallel and perpendicular direction to the plane of incidence, is reflected on the surface of water contained in a vat. It is shown that this model is capable of predicting the disappearance, during the afternoon, of a coin contained in a vessel with a little water and illumintated by sunlight. We understand that this article contributes to the teaching of Physics, as it brings an activity that promotes the connection between theoretical and practical knowledge from a potentially exciting problem, aiding students to build meaning from scientific knowledge and understand central elements of the experimental work from the perspective of scientific modeling.